Micro-Manager Metadata Viewer
Posted by LC | Filed under image, tip
This post title is very self-explained.
You can check it out from my gist mm-json.html.
Arduino UNO control OBIS under its Digital Trigger mode
Posted by LC | Filed under tip
It is done. With less modules controlled by Micro-Manager, the system loads faster and more stable.
Notes during the whole process:
- coherent OBIS use SMB but not BNC connectors
- disable "5-second CDRH delay" and swithc operation mode to "Digital Power" in Coherent Connection software
- Follow the following two posts exactly, setup Arduino in Micro-Manager and connect the laser to Arduino ...
crispr scanner
Posted by LC | Filed under tip
First, get ots_server running, which is from htgt/CRISPR-Analyser. Install Web Blat is recommended but not required.
Second, download crispr.html from my Gist, and download a copy of jquery-2.1.1.min.js. Put both files in the same folder.
Third and final step, open crispr.html with a modern browser (no IE<9 support).
Enjoy!
Above is an ...
看文献的个人体会
Posted by LC | Filed under tip
1，看Figures和Results
因为这部分是就事论事，一个图得出一个结论。按照惯例，这部分不应该含任何的讨论和深入。图例有时会有不少实验细节，可能需要看Experimental Procedures帮助理解。
2，看Introduction
通过阅读按照作者的思路介绍的背景知识，加深对刚刚阅读的结果的理解。可能需要根据作者引用的文章，适当选择阅读，加深理解。
3，看Discussion
这是作者对本文章的讨论和深入，阅读需要一定的背景知识。由于文章Discussion的最后一段是总结发现，阐明意义；我一般是先读最后一段，把握基调，然后读整个Discussion。
- 粗读，对我来说就是看Figures和图例；有兴趣再读Results
- 细读，我会看Figures和图例，Results和Introduction；快速扫一下Discussion，有兴趣才会多看一下
- 精读，就是1-2-3，然后中间多次往返各个部分；一般在一次阅读后，过段时间，会再看看它的Discussion，思考思考
what should be put into the lab notebook
Posted by LC | Filed under tip
Keep all your notebooks, which might be important supporting evidence after many years
Keep every page of the notebook
Do not write on the notebook with pencil, or any other means which can be easily erased
The first 2~4 pages of the notebook should be used as the reference points to plasmid design pages, important experiments, etc
Only put ...