what should be put into the lab notebook
Posted by cai-lab | Filed under lab
still a work in progress
Keep every page of the notebook
The first 2-4 pages of the notebook use as reference point to plasmid design page, important experiment note, etc
Put short code explanation in the first several pages
For imaging experiments using microscopy: create individual folder for every experiment, with year-month-day as part of the folder name; I like ...
fix and stained cells on 2D surface
Posted by cai-lab | Filed under image
These images are ANCIENT~~
lab policies
Posted by cai-lab | Filed under lab
adapted from another lab
All lab members are expected to attend the weekly lab meeting and occasional special meetings, such as meeting reviews and invited talks, on time. If you miss lab business, you are still responsible for items that were discussed. All lab meeting presentations should be placed on the server in appropriate folders.
Please diligently perform your assigned ...
expectations from new members
Posted by cai-lab | Filed under lab
All new members are expected to finish the following tasks in a month (under the supervision of the PI)
prepare a batch of chemical competent cells (competence >1E+8)
make a new construct by PCR based cut-paste approach and sequencing verified (including designing primers, PCR, mini-prep, DNA digestion, DNA gel electrophoresis, etc)
make an RNAi construct and sequencing verified (including ...