expectations from new members
Posted by LC | Filed under lab
All new members are expected to finish the following tasks in a month (under the supervision of the PI)
prepare a batch of chemical competent cells (competence >1E+8)
make a new construct by PCR based cut-paste approach and sequencing verified (including designing primers, PCR, mini-prep, DNA digestion, DNA gel electrophoresis, etc)
make an RNAi construct and sequencing verified (including oligo design)
purify a batch of GST fusion protein using an existing bacteria line
culture some fibroblasts, plate them on coverslips, fix and stain with some antibodies, mount to slides
image your stained samples with epi-fluorescence and process it using Fuji