expectations from new members

All new members are expected to finish the following tasks in a month (under the supervision of the PI)

  • prepare a batch of chemical competent cells (competence >1E+8)

  • make a new construct by PCR based cut-paste approach and sequencing verified (including designing primers, PCR, mini-prep, DNA digestion, DNA gel electrophoresis, etc)

  • make an RNAi construct and sequencing verified (including oligo design)

  • purify a batch of GST fusion protein using an existing bacteria line

  • culture some fibroblasts, plate them on coverslips, fix and stain with some antibodies, mount to slides

  • image your stained samples with epi-fluorescence and process it using Fuji