the lab has many things in it now

Now waiting for the cells, and the people back from the summer vacation...

image on actin.cn

the empty temporarily lab space

This space is being used until the new building in Jiangwan is ready.

The following photos were taken a month ago, and posted here on 6-7-2012.

image on actin.cn

看文献的个人体会

1,看Figures和Results

因为这部分是就事论事,一个图得出一个结论。按照惯例,这部分不应该含任何的讨论和深入。图例有时会有不少实验细节,可能需要看Experimental Procedures帮助理解。

2,看Introduction

通过阅读按照作者的思路介绍的背景知识,加深对刚刚阅读的结果的理解。可能需要根据作者引用的文章,适当选择阅读,加深理解。

3,看Discussion

这是作者对本文章的讨论和深入,阅读需要一定的背景知识。由于文章Discussion的最后一段是总结发现,阐明意义;我一般是先读最后一段,把握基调,然后读整个Discussion。

  • 粗读,对我来说就是看Figures和图例;有兴趣再读Results
  • 细读,我会看Figures和图例,Results和Introduction;快速扫一下Discussion,有兴趣才会多看一下
  • 精读,就是1-2-3,然后中间多次往返各个部分;一般在一次阅读后,过段时间,会再看看它的Discussion,思考思考

what should be put into the lab notebook

  • Keep all your notebooks, which might be important supporting evidence after many years

  • Keep every page of the notebook

  • Do not write on the notebook with pencil, or any other means which can be easily erased

  • The first 2~4 pages of the notebook should be used as the reference points to plasmid design pages, important experiments, etc

  • Only put ...

fix and stained cells on 2D surface

These images are ANCIENT~~

1 cell