看文献的个人体会

1,看Figures和Results

因为这部分是就事论事,一个图得出一个结论。按照惯例,这部分不应该含任何的讨论和深入。图例有时会有不少实验细节,可能需要看Experimental Procedures帮助理解。

2,看Introduction

通过阅读按照作者的思路介绍的背景知识,加深对刚刚阅读的结果的理解。可能需要根据作者引用的文章,适当选择阅读,加深理解。

3,看Discussion

这是作者对本文章的讨论和深入,阅读需要一定的背景知识。由于文章Discussion的最后一段是总结发现,阐明意义;我一般是先读最后一段,把握基调,然后读整个Discussion。

  • 粗读,对我来说就是看Figures和图例;有兴趣再读Results
  • 细读,我会看Figures和图例,Results和Introduction;快速扫一下Discussion,有兴趣才会多看一下
  • 精读,就是1-2-3,然后中间多次往返各个部分;一般在一次阅读后,过段时间,会再看看它的Discussion,思考思考

what should be put into the lab notebook

  • Keep all your notebooks, which might be important supporting evidence after many years

  • Keep every page of the notebook

  • Do not write on the notebook with pencil, or any other means which can be easily erased

  • The first 2~4 pages of the notebook should be used as the reference points to plasmid design pages, important experiments, etc

  • Only put ...

fix and stained cells on 2D surface

These images are ANCIENT~~

1 cell

lab policies

All lab members are expected to attend the weekly lab meeting and occasional special meetings, such as meeting reviews and invited talks, on time. If you miss lab business, you are still responsible for items that were discussed. All lab meeting presentations should be placed on the server in appropriate folders.

实验室所有成员都要准时参加每周一次的组会和不定期的特别会议(例如,工作总结会和邀请报告)。如果你错过了实验室职责分工的讨论(一般每学期一次),你仍需要负责所指派的项目。所有的会议汇报应放在服务器上相应的文件夹 ...

expectations from new members

All new members are expected to finish the following tasks in a month (under the supervision of the PI)

  • prepare a batch of chemical competent cells (competence >1E+8)

  • make a new construct by PCR based cut-paste approach and sequencing verified (including designing primers, PCR, mini-prep, DNA digestion, DNA gel electrophoresis, etc)

  • make an RNAi construct and sequencing verified (including ...